Journal: Respiratory Research

Article Title: Activation of PBK by Gal-3 contributes to pulmonary artery hypertension by promoting PRC1 activation

doi: 10.1186/s12931-026-03534-4

Figure Lengend Snippet: Relevant interventions attenuate the development of MCT-induced PAH rat. A Comparison of mPAP ( n = 6). B Comparison of RVSP ( n = 6). C Comparison of RV/ (LV + S) ( n = 4). D Quantification of wall thickness ( n = 4). E Quantification of muscularization ( n = 4). F HE staining reveals right ventricular hypertrophy, scale bar = 5000 μm; HE staining and EVG staining reflect medial wall thickness, scale bar = 50 μm; muscularization revealed by immunohistochemical staining of α-SMA, scale bar = 100 μm. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: PASMCs within six passages were utilized, and immunofluorescence staining with α-SMA (Proteintech, Wuhan, China, 14395-1-AP) was used to verify the cells contained over 95% PASMCs.

Techniques: Comparison, Staining, Immunohistochemical staining

Journal: Journal of ethnopharmacology

Article Title: Pretreatment with the active fraction of Rhodiola tangutica (Maxim.) S.H. Fu rescues hypoxia-induced potassium channel inhibition in rat pulmonary artery smooth muscle cells.

doi: 10.1016/j.jep.2021.114734

Figure Lengend Snippet: Fig. 1. ACRT inhibits rat PASMC proliferation. (A) Morphology of rat PASMCs isolated from rat pulmonary arterioles. (B) Rat PASMCs were identified by immunohistochemistrical analysis. Rat PASMCs were stained with α-SMA after incubation. (C) ACRT inhibits PASMC proliferation (n = 6). Rat PASMCs were treated with different concentrations of an ACRT solution under hypoxic con ditions (3% O2) for 24 or 48 h. Cell viability was examined using the CCK- 8 assay.

Article Snippet: Rat PASMCs were isolated and identified by anti-α-smooth muscle actin (α-SMA) antibody (Boster, Wuhan, China).

Techniques: Isolation, Staining, Incubation, CCK-8 Assay